Ghasem Ghorbani Nasrabadi; Esmaeil Dordipour; mojtaba Barani; Elham Malekzadeh; Abdolreza Gharanjiki
Abstract
Introduction Salinity is one of the most important environmental stresses limiting agricultural production in arid and semi-arid regions, which occupies a relatively large area of arable land. Nutrient availability is decreased in saline conditions in soil solution and plant nutrient balance is changed. ...
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Introduction Salinity is one of the most important environmental stresses limiting agricultural production in arid and semi-arid regions, which occupies a relatively large area of arable land. Nutrient availability is decreased in saline conditions in soil solution and plant nutrient balance is changed. Nitrogen fertilizer management as an essential nutrient for plant growth is very important under salinity stress. Also, salicylic acid is a plant growth regulator involved in defense mechanisms of plants against biotic and abiotic stresses. Therefore, the aim of this study was to investigate the effect of salinity on the concentration of nutrients in wheat in response to salicylic acid consumption at different levels of nitrogen.Materials and Methods In order to investigate the effects of salicylic acid and nitrogen fertilizer application rates at different salinity levels on nutrient concentration of wheat cv. Morvarid, an experiment was conducted as a split plot factorial based on a randomized complete block design with four replications in the fields of Mazraeh-E-Nemooneh located in Anbarolum, Aq Qala city, Golestan province. The main factor included three soil salinity levels (3-4 below wheat tolerance threshold (control), 9-11 and 13-15 dS.m-1) and sub factors included two levels of salicylic acid (0 and 1.5 mM) and three levels of N fertilizer (from urea source, 46% N) were 1) N based on soil test recommendation, 2) 30% N more than soil test recommendation and 3) 30% N less than soil test recommendation, respectively. Salicylic acid was foliar applied twice for about 2 weeks in the tillering stage and 10 days after the second stage spraying, content in them was determined. Nitrogen treatments were applied in three stages - one third before planting with ammonium sulfate (21% N) and remains top-dressed with urea (46% N) at tillering and stem elongation stages. At the emergence stage of the cluster or the beginning of flowering of wheat, the amount of nitrogen in the flag leaf was measured. The concentrations of nitrogen, potassium and sodium in grain and straw were also measured by standard methods.Results and Discussion The results showed that by increasing salinity, the flag leaf N concentration, N and K concentration of wheat staw and seed decreased. However, Na concentration of straw and seeds increased. With increasing N and salicylic acid consumption, the concentration of N flag leaf, the seed and straw N and K concentrations increased, but the concentration of Na in seed and straw decreased.The interaction of salinity, salicylic acid and nitrogen on seed N and K concentrations and also on flag leaf N concentration was significant but there was no significant effect on other measured elements. Comparison of the mean of simple effects of salinity on the evaluated elements indicates a significant reduction of all studied elements due to salinity treatment. Also, comparison of the mean of simple effects of nitrogen fertilizer showed that all elements were affected by the treatment. The results of mean comparison showed a positive and significant effect of salicylic acid on the leaf N concentration of the flag leaf, so that the N concentration in the flag leaf in a 1.5 mM salicylic acid treatment was significantly increased compared to the non-use treatment.Conclusion according to the results, more nitrogen consumption at moderate salinity can have a positive effect on plant nutrition, and vice versa, at high salinity levels, it is better to reduce nitrogen consumption. The interactions of salicylic acid and nitrogen showed that in general nitrogen treatments with salicylic acid increased the N concentration of seed and straw. Nitrogen fertilization at higher and medium salinity levels increased the concentration of N and K in straw and seeds; However, at high salinity, less nitrogen fertilization improved the concentration of N and K. Also, nitrogen application with salicylic acid improved these traits under saline conditions. Therefore, the application of salicylic acid and nitrogen fertilizer management to some extent reduced the adverse effects of salinity up to moderate salinity levels and improved plant nutrition by increasing plant tolerance to salinity.Therefore, the application of salicylic acid and nitrogen fertilizer management to some extent reduced the adverse effects of salinity up to moderate salinity levels and improved plant nutrition by increasing plant tolerance to salinity.Key words: Salicylic acid, salinity stress, wheat, Nitrogen fertilizer management
Soil Chemistry and Pollution
Narges sousaraeS; Mojtaba Baranimotlagh; Farhad Khormali; Esmaeil Dordipour
Abstract
Introduction Biochar is a charcoal, pyrolyzed from a wide range of carbon-rich biomass materials, such as crop and wood residues, animal manures and a range of industrial wastes and once added into soil, it can store the soil carbon for a long period, improve the soil structure and increase the crop ...
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Introduction Biochar is a charcoal, pyrolyzed from a wide range of carbon-rich biomass materials, such as crop and wood residues, animal manures and a range of industrial wastes and once added into soil, it can store the soil carbon for a long period, improve the soil structure and increase the crop yield. However, the physical and chemical characteristics of biochars are influenced by the properties of the feedstock and pyrolysis conditions, such as highest temperature treatment and furnace residence time. Considering the large variation in biochar properties, it is not surprising that crop yields vary with different biochars. We investigated the effects of biochars on corn growth in the greenhouse. The specific objectives were (a) to assess whether feedstock properties or pyrolysis temperature are important in preparing of biochar and (b) to quantify the effects of varying biochar characteristics on corn growth and chlorophyll index in a calcareous soil under greenhouse condition. Materials and Methods Biochar was produced from crop residues including rice, cotton and canola. Feedstock was oven-dried before pyrolysis. The pyrolysis process was conducted for 1 h at 10°C min‒1 heating rate to produce biochars at different temperatures of 350 and 700 oC under oxygen-limited conditions. All biochars were ground and passed through a 2-mm sieve before experimentation. Ash content and char yield was calculated and biochar pH and electrical conductivity (EC) were measured using 1:20 solid: solution ratio. The soil used in this experiment was taken from the Research Farm of Gorgan University of Agricultural Sciences and Natural Resources. The soil was air-dried and ground to pass through a 2-mm sieve then analyzed for various soil physico-chemical properties using standard methods. A greenhouse experiment was set up using pots with 5 kg prepared soil. Various treatments comprising of 3 biochars type produced at different pyrolysis temperatures (350 and 700°C) from three crop residues (rice, cotton and canola) at three application rates (0, 2 and 5% w/w). A completely randomized design was used in factorial arrangement and treatments were replicated four times. After the soil had been prepared and biochar added, six seeds of maize were planted approximately 20 mm deep in the center of the pots and thinning to seedlings of four plants pot‒1 was done at plant establishment. Distilled water was used to maintain moisture contents of the soil in all the pots during the experimental period. Plant stem and leaves were harvested 96 days after planting. Washed with distilled water then dried with tissue paper. The leaf and stem samples were air-dried and then oven dried at 65˚C to a constant weight in a forced air driven oven. The studied traits included leaf and stem fresh and dry weight, plant height, number of leaves, time to first flowering, chlorophyll index (SPAD), concentration of chlorophyll a, chlorophyll b and total chlorophyll. The analysis of variance (ANOVA) with the factors biochar type, application rate and pyrolysis temperature were performed using a completely randomized design. Significantly different treatment means were separated using least significant difference (LSD) test at PResults and Discussion The results showed that pyrolysis temperature significantly influenced the measured chemical properties of biochars. EC values were tended to increase with pyrolysis temperature. The pH of the biochars was also influenced by temperature. Biochars pH ranged from 6.8 to 9.6. The pH of the biochars was increased with increasing temperature and highest pH (9.6) was observed at 700°C of rice residues. These increases in pH values are mainly due to separating of alkali salts from organic materials by increased pyrolysis temperature. The results showed that the yield of biochars was reduced by increasing pyrolysis temperature and ranged from 19.4% to 40.1%. This decline in yield content is mainly due to the destruction of some compounds such as cellulose and hemicellulose as well as combustion of organic materials with increased pyrolysis temperature. By contrast to biochar yield, the biochar ash content increased with increasing pyrolysis temperature. The lowest values of leaf and stem fresh and dry weight was observed at 700°C of canola residues. These results suggest that biochar produced at high pyrolysis temperature (especially at 700°C), when applied to the soil, may increase soil salinity and subsequently provide undesirable impacts on the plant growth. It has been reported that the negative impacts of high salinity on the plant growth could be due to the following reasons: (1) the low osmotic potential of the soil solution, resulting in water stress, (2) specific ion effects, resulting in salt stress, and (3) nutrient imbalances. Addition of each three types of biochars caused a significant increase in chlorophyll concentration compared to control. Conclusion The type of feedstock material is an important factor that determines the final application of the biochar and its effect on plant growth papameters. Therefore, there is further need for research focusing on the effects of biochar addition on soil properties and plant growth in order to assess biochar as a valuable resource for agriculture.
Plant Nutrition, Soil Fertility and Fertilizers
Esmaeil Dordipour; Zeinab Bastamikojour; Mojtaba Baranimotlagh; Abdolreza Gharanjiki; Mohsen Olamaee
Abstract
Introduction The most important constraint in maize crop yield in developing contries worldwide, and especially among resource-poor farmers, is soil infertility. Therefore, maintaining soil quality can reduce the problems of land degradation that decreases soil fertility and rapidly declining production ...
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Introduction The most important constraint in maize crop yield in developing contries worldwide, and especially among resource-poor farmers, is soil infertility. Therefore, maintaining soil quality can reduce the problems of land degradation that decreases soil fertility and rapidly declining production levels that occur in large parts of the world which needing the basic principles of good farming practice. For optimum plant growth, nutrients must be available in sufficient and balanced quantities. After nitrogen, phosphorus is the most limiting nutrient for crop yields, and is essential for maize growth and development. Large quantities of chemical fertilizers are used to replenish soil N and P, resulting in high costs and severe environmental contamination. Maize quantity and quality are increased by utilization of fertilizers, which has become the most important objective of these products worldwide. Phosphorus, is the second most important macronutrient required by the plants, next to nitrogen, and is reported to be a critical factor of many crop production systems due to its limited availability in soluble forms in the soils. The low availability of P to plants is because the vast majority of soil P is found in insoluble forms, and plants can only absorb P in two soluble forms, the monobasic (H2PO4-) and the dibasic (HPO42-) ions. Crop plants can therefore utilize only a fraction of applied phosphorus, which ultimately results in poor crop performance. To rectify this and to maintain soil fertility status, frequent application of chemical fertilizers is needed, though it is found to be a costly affair and also environmentally undesirable. Moreover, phosphorus (P) is an essential nutrientionl element for plant growth. Calcareous soils are frequently characterized by the low availability of P for plant uptake due to the low solubility of P compounds present in soils at high pH and the formation of relatively insoluble complexes, e.g., Ca-P. Many soils in Iran have received large amounts of P fertilizer and consequently contained a high level of available P. On the other hand, the root exudation of organic acids has been suggested to increase P availability in calcareous soils. The most common low-molecularweight organic acids (LMWOAs) identified in soils include oxalic, succinic, tartaric, fumaric, malic, and citric acids and are derived from the decomposition of soil organic matter in the upper soil horizons, microbial metabolites, canopy drip, and root exudation. The concentrations of organic acids in the rhizosphere or in soil solutions vary greatly and range from 10-2μM to over 80 mM. The ability of organic acids to release inorganic anions, such as P, has been reported and has been attributed to desorption of inorganic anions and solubilization of phosphate compounds. LMWOAs and their corresponding anions play a very important role to increase P bioavailability. Many studies have been conducted about the role of organic acids in increasing P availability, but these studies focused on acid soils in which Fe- or Al-bound P is the main P fraction. For calcareous soils where Ca-bound P is the main P fraction, questions that whether organic acids can mobilize P or not still exist. Although, a number of results show that addition of organic acids, especially citric and oxalic acids to soils can solubilize significant quantities of fixed P and reduce the sorption of newly applied fertilizer P. However, there are few studies on the transformations of P fractions induced by organic acids or organic anions, which are important for understanding the mobilization mechanisms of P and for exploring better ways of using different forms of P in soils. The objective of this study is to examine the effects of some organic acids and anions on the solubilization and plant uptake of soil P in some calcareous soils of Golestan province, Iran. Material and Methods For this purpose, a factorial pot experiment in a completely randomized design with three replications was conducted on maize. The first factor was comprised of 6 soil types from various areas of the province and the second factor was consisted of a combination of phosphorus fertilizer and organic acids (1) control, (2) 50 mg P kg-1, (3 and 4) 50 mmol kg-1 of organic acids (oxalic and malic acids), (5) P + oxalic acid and, (6) tomato fruit residue (25% w). After 10 weeks, plants were harvested and the parameters such as plant height, fresh and dry weights, phosphorus concentration and its uptake were determined. Results and Discussion Results indicated that soil type effect was statistically significant on the plant fresh and dry weights (P≤ 0.05), height, concentration and uptake of P (P≤ 0.01), respectively. Results also showed that the tomato fruit residue treatment in comparison with P fertilizer and malic acid treatments results in a significant increase in P taken up, and fresh and dry weights (P≤ 0.05). There was a significant difference between P fertilizer + oxalic acid and oxalic acid alone treatments in only plant height (P≤ 0.05). Also, no significant differences in terms of measured plant parameters were observed between malic acid and blank treatments (P≤ 0.05). Conclusion Application of tomato fruit residue rather than P fertilizer can help to take up residual soil P, to grow plants and to decrease of environmental pollution, and to be also affordable economically.
